Human Anti-SARS CoV-2 Nucleocapsid Protein (HuN4) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：Recombinant human monoclonal antibody against SARS-CoV-2 Nucleocapsid protein, produced via Agrobacterium tumefaciens

                      infiltration of Nicotiana benthamiana plants.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 500 – 1: 2 000) ELISA (1: 1 000 – 1: 4 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1 M Phosphate Buffered Saline, pH 7.4.Preservative: none

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95 % as determined by SDS-PAGE.

99.30% as determined by Mass Spectrometry.

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of Human Anti-SARS-CoV-2 N (HuN4) Antibody, used to detect 1 ng/µl of SARS-CoV-2 Nucleocapsid Phosphoprotein.

Figure 2. Western blot analysis of Human Anti-SARS-CoV-2 N (HuN4). Lanes 2 – 5: Varying amounts of SARS-CoV-2 Nucleocapsid (N) Phosphoprotein were run on the SDS-PAGE. Separated bands were transferred to the membrane and Human Anti-N (HuN4) (1: 1 000) was used to detect the antigen. The SARS-CoV-2 N Phosphoprotein bands (~46 kDa) were visualized in each lane following the addition of anti-human secondary antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 15.6 ng (Lane 5).