Human Anti-SARS CoV-2 Nucleocapsid Protein (N12.8) (HRP) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype： Human IgG1

Description：Recombinant human monoclonal antibody against SARS-CoV-2 Nucleocapsid protein, fused at the genetic level to HRP reporter 

                      enzyme. This protein was produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 500 – 1: 1 000) ELISA (1: 1 000 – 1: 15 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ：Liquid

Storage： Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1 M Phosphate Buffered Saline, pH 7.4.Preservative: 50% Glycerol

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 90 % as determined by SDS-PAGE

94.20% as determined by Mass Spectrometry.

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve showing Human Anti-N (N12.8) (HRP) Antibody used from 0,003 – 50 ng/uL to detect 50 ng SARS-CoV-2 Nucleocapsid protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot showing decreasing amounts of SARS-CoV-2 Nucleocapsid protein (46 kDa) detected with Human Anti-N (N12.8) (HRP) Antibody at 1:1000.