Mouse Anti-Delta-9-Tetrahydrocannabinol Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description：This product is a full-length Mouse IgG1 antibody specific to the Delta-9-Tetrahydrocannabinol (THC) molecule. It was produced 

                      recombinantly in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： Western blot (1: 1 000 – 1: 8 000)

ELISA (1: 10 000 – 1: 100 000)

Tested Species Reactivity ： Human

Concentration ：1 mg/ml

Form ： Liquid

Storage： Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1   M Phosphate Buffered Saline, pH 7.8. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. Western blot of decreasing amounts of THC-BSA antigen (73 kDa) detected using Mouse Anti-Delta-9-THC at 1: 5 000 and an HRP conjugated anti-mouse secondary antibody.

Figure 2. ELISA Dose Response curve using Mouse Anti-Delta-9-THC to detect coated THC-BSA Antigen at 0.1 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).