Rabbit Anti-CD20 (1F5) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype：Rabbit IgG1

Description：This product is a full-length Mouse IgG2a recombinant antibody specific to Human B-lymphocyte antigen CD20. It was produced in

                      Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： ELISA (1: 1 000 – 1: 5 000)

Western blot (1: 500 – 1: 1 000)

Tested Species Reactivity ： Human

Concentration ： 1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1   M Phosphate Buffered Saline, pH 7.7. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve: using Rabbit Anti-CD20 (1F5) Antibody from 20-0,0026 ng/uL to detect 50 ng Human B-lymphocyte antigen CD20 antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human CD20-His-Avi Tag (~16.8 kDa) detected with Rabbit Anti-CD20 (1F5) at 1: 1 000 and an HRP conjugated anti-Rabbit secondary antibody at 1:10 000.