Mouse Anti-Alpha Tubulin (F2C) Recombinant Antibody

Expression Host：Nicotiana benthamiana plants

Clonality： Monoclonal, recombinant

Species and Isotype： Mouse IgG2a

Description： This product is a full-length Mouse IgG2a recombinant antibody specific to Alpha Tubulin. It was produced in 

                       Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range： EWestern blot (1: 1 000 – 1: 5 000) ELISA (1: 5 000 – 1: 450 000)

Tested Species Reactivity ： Human, Mouse

Concentration ：1 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer： 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None

Purification Notes： This product was purified using Protein A- affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes：For Research Use only, unless otherwise indicated.

Image：

Figure 1. ELISA Dose Response curve using Mouse Anti-Alpha Tubulin (F2C) to detect coated Alpha 1a Tubulin antigen at 0.25 ng/µl 

and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Alpha 1a Tubulin antigen (~50 kDa) detected using Mouse Anti-Alpha Tubulin (F2C) at 1: 10 000.

Figure 3. ELISA Dose Response curve using Mouse Anti-Alpha Tubulin (F2C) to detect coated Alpha 1a Tubulin Antigen stored at -20°C and

shipping conditions for two weeks. At shipping the product is placed under conditions that simulate real time shipping conditions.

 Experiments were performed in triplicate, with error bars representing standard deviation (SD).