Human Anti-His tag (C-term) (3D5) Recombinant Antibody (HRP)

Expression Host：Nicotiana benthamiana plants

Clonality：Monoclonal, recombinant

Species and Isotype：Human IgG1

Description：This product is a full-length Human IgG1 antibody specific to Polyhistidine tag/ purified his-tagged Fab fragment 

                     at the carboxy-terminal molecule. It was produced recombinantly in Nicotiana benthamiana plants via Agrobacterium

                     tumefaciens mediated infiltration.

Verified Applications：Western blot, ELISA

Dilution Range：Western blot (1: 500 -1: 5 000)

ELISA (1: 1 000 – 1: 150 000)

Tested Species Reactivity ： Human

Concentration ：0.5 mg/ml

Form ：Liquid

Storage：Short-term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze-thaw cycles.

Storage Buffer：0.1  M Phosphate Buffered Saline, pH 7.4

Preservative: 25% Glycerol

Purification Notes：This product was purified using Protein A affinity chromatography.

Purity：≥ 95% as determined by SDS-PAGE

General Notes： For Research Use only, unless otherwise indicated.  

Image：

Figure 1. Western blot of decreasing amounts of S1-His antigen (73 kDa) detected using Human Anti-His tag (C-term) (3D5) (HRP) at 1: 5 000.

Figure 2. ELISA Dose Response curve using Human Anti-His tag (C-term) (3D5) (HRP) to detect coated S1-His Antigen at 1 ng/µl and 

increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).