18502057686
种属:人
别称:SNF96.2; sNF96-2
组织来源:施万细胞
疾病:1型神经纤维瘤病Nf1
传代比例/细胞消化:1:2传代,
完全培养基配置:DMEM培养基 ;10%胎牛血清 ;1%双抗
简介:sNF96.2 is a Schwann cell line that was isolated in 1996 from a 28-year-old, male patient with neurofibromatosis type 1 Nf1.The
sNF96.2 cell line was derived from a recurrent mass associated with nerve and diagnosed as MPNST (Malignant Peripheral Nerve Sheath
Tumor) in a patient meeting NF1 diagnostic criteria. The cells were derived from numerous passages of primary tumor material in culture
until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic
Schwann cell markers S100 and p75.
形态:雪旺细胞样
生长特征:贴壁生长
STR:Amelogenin X CSF1PO 12 D5S818 11 D7S820 10,11 D13S317 10 D16S539 11 TH01 6 TPOX 11
vWA 17,19
倍增时间:~33 h
培养条件:气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。
保藏机构:ATCC; CRL-2884
冻存条件:无血清冻存液:官网货号KF-H0003
仅供科研或生产使用,不可直接应用于人体。
注意:
1:观察有无破损漏液情况,如有请拍照及时联系客服。
2:酒精消毒培养瓶表面后显微镜下观察细胞状态,观察拍照后不用打开培养瓶盖
放入培养箱静止2-3小时稳定 细胞状态。
3:产品随货会附带细胞说明书、细胞培养操作指南、细胞鉴定、支原体检测报告。
4:若产品有异常或其他疑问,可随时联系客服;转至技术支持。
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