本司产品仅用于科研,不用于临床诊断和治疗

人脊索瘤细胞U-CH1(STR鉴定正确)

货号:KF-0831


规格:1×10⁶cells/T25培养瓶
¥2900.00

种属: 人

别称: UCH-1; UCH1

组织来源: 脊柱;骶骨

疾病: 脊索瘤

传代比例/细胞消化:1:2传代 ,消化2-3分钟

完全培养基配置: 80% mixture of Iscove's MDM and RPMI 1640 (at 4:1);10%胎牛血清; 1%双抗

背景描述: U-CH1 is the first human chordoma cell line. It exhibits  chordoma-like characteristics and has molecular, genetic, and  morphological features typical of chordoma. This cell line was  established from a local recurrence of a sacrococcygeal chordoma  after radiotherapy 4 years after initial surgery. Chordoma is a  rare slow-growing tumor type, and U-CH1 is a relatively  slow-growing cell line. U-CH1 has a heterogeneous morphology  consisting of physaliferous cells with mcinous intercellular

substance, which represent typical chordoma features. The cells  overexpress the transcription factor T (Brachyury) that is the most  specific marker for chordoma. This cell line was accessioned with  the support of the Chordoma Foundation, a non-profit organization  working to improve the lives of chordoma patients by accelerating  research to develop effective treatments for the chordoma disease.

形态:  间充质细胞样

生长特征:  贴壁生长

STR:  Amelogenin X,Y CSF1PO 10,11 D1S1656 16,16.3 D2S441 12,14 D2S1338  17,18 D3S1358 15 D5S818 11,12 D7S820 9,12 D8S1179 10,15 D10S1248  15 D12S391 17,22 D13S317 11,13 D16S539 12,13 D18S51 15 D19S433 14  D21S11 28,29 D22S1045 16 FGA 20,21 Penta D 11 Penta E 7,10 TH01  7 TPOX 8,11 vWA 17

倍增时间: 每周 2 至 3 次

培养条件:  气相:空气 ,95% ;二氧化碳 ,5%。 温度:37摄氏度 ,培养箱湿度 为70%-80%。

保藏机构: ATCC; CRL-3217

冻存条件:  无血清冻存液:官网货号KF-H0003

仅供科研或生产使用,不可直接应用于人体。

 

注意:

1:观察有无破损漏液情况,如有请拍照及时联系客服。

2:酒精消毒培养瓶表面后显微镜下观察细胞状态,观察拍照后不用打开培养瓶盖

放入培养箱静止2-3小时稳定 细胞状态。

3:产品随货会附带细胞说明书、细胞培养操作指南、细胞鉴定、支原体检测报告。

4:若产品有异常或其他疑问,可随时联系客服;转至技术支持。


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